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Cholera toxin B subunit was coated on ELISA plates and probed with individual heat‐inactivated sera samples (starting with a 1 : 50 dilution). CTB ‐IgG1 antibody titres at different time points: (a, b) …

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Porcine epidemic diarrhea virus (PEDV) belongs to the Coronaviridae family and causes acute enteritis in pigs. A fragment of the large spike glycoprotein, termed the S1D epitope (aa 636–789), alone and fused with cholera toxin B subunit, were independently cloned into plant expression vectors, yielding plasmids pMYV717 and pMYV719, respectively.

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Porcine epidemic diarrhea virus (PEDV) belongs to the Coronaviridae family and causes acute enteritis in pigs. A fragment of the large spike glycoprotein, termed the S1D epitope (aa 636–789), alone and fused with cholera toxin B …

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Therefore, exendin-4 (EX4) was expressed as a cholera toxin B subunit (CTB)-fusion protein in tobacco chloroplasts to facilitate bioencapsulation within plant cells and transmucosal delivery in the gut via GM1 receptors present in the intestinal epithelium. The transgene integration was confirmed by PCR and Southern blot analysis.

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The designed plasmid maps are shown in Fig. 1A and B. It was observed that the target proteins were induced to express EGF-CTA 2-TAT (15.7 kDa) and CTB (12.8 kDa) (Fig. 1C and D). The purity of the products was verified by western blotting with an anti-CTB antibody and an anti-EGF-CTA 2-TAT antibody (Fig. 1E). The CT-like chimeric protein was obtained by acid-base renaturation of CTB and …

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